Light sheet microscopy incorporating adaptive optics
Lawrence, Keelan Michael
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When imaging deep into biological samples in Light Sheet Microscopy, refractive index variations within the sample, as well as differences between the sample, immersion medium, and sample holder distort the optical wavefront causing a loss of resolution and a decrease of the signal to noise ratio of the image. By implementing Adaptive Optics in a light sheet system, optical aberrations can be corrected and resolution can be increased despite the wavefront distortions cause by the organism. Adaptive Optics systems operate by sensing the wavefront of the incident light and then correcting the distortions in the wavefront with a modulation element. In this paper, we cover the design and implementation of an Adaptive Optics system composed of a deformable mirror and Shack-Hartmann Wavefront Sensor for the measurement and correction of wavefront aberrations caused by zebrafish (D. rerio) larvae during live imaging with Light Sheet Microscopy.