Glucose concentration affects fibrin clot structure:
Hood, Jacob Edward
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Hyperglycemia’s influences on unstable and irregular clot formation in diabetes mellitus (DM) remains unclear because many factors known to influence fibrinogen to fibrin conversion, clot morphology, and thrombus formation present simultaneously in these patients. An LSM 880 (Laser Scanning Microscope) was used to analyze the effects of glucose concentration on fibrin clot structure; fibrinogen glycation was conducted to support characteristic findings. Algorithm outputs supported in vivo work and found that hyperglycemia, via increased fibrinogen glycation, alters fibrin clot structure at a fixed fibrinogen concentration. Additionally, 10 wt% S-Nitroso-Nacetyl-D-penicillamine (SNAP) was investigated to determine the efficacy in utilizing nitric oxide (NO) mechanisms to limit clot formation in hyperglycemic environments. Four 10 wt% SNAP polymer exposure conditions resulted in no polymerization and was likely a result of increased protein adhesion to the polymer film. NO was believed to further increase protein adhesion to the polymer as incubation with 10 wt% SNAP reduced dyed fibrinogen concentration significantly more (72.36%) than the non-NO releasing control.