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dc.contributor.authorCai, Wenjie
dc.date.accessioned2018-07-31T04:30:13Z
dc.date.available2018-07-31T04:30:13Z
dc.date.issued2018-05
dc.identifier.othercai_wenjie_201805_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/cai_wenjie_201805_ms
dc.identifier.urihttp://hdl.handle.net/10724/38348
dc.description.abstractAflatoxins (AF) are group I human carcinogen and potent mycotoxins that commonly contaminate peanuts, corns, and their products during growth, harvest and storage. Exposure to AF is linked to growth stunting in children, which has been extensively studied in recent years. With increasing involvement of young children as study subjects, dried blood spot (DBS) rose in popularity as an important alternative blood collection strategy. In this study, an HPLC-fluorescence method measuring exposure biomarker of AFB1, AFB1-lysine adduct, was developed and validated. For initial method development and validation, DBS and serum samples were obtained from AFB1-treated F344 rats, as well as selected samples from human field study, which were analyzed and compared, and has found good correlation between serum and DBS samples (0.997 for the single-dose exposure and 0.996 for repeated dose exposure). DBS samples (n=393) from Nepal were analyzed and compared with the results from matching serum samples. The result suggested that the HPLC-florescence method is effective in detecting AFB1-lysine adducts and can potentially be applied in epidemiological studies.
dc.languageeng
dc.publisheruga
dc.rightsOn Campus Only Until 2020-05-01
dc.subjectAflatoxin B1
dc.subjectdried blood spots
dc.subjectHPLC-fluorescence
dc.titleAnalysis of aflatoxin B1-lysine Adduct in infant dried blood spot samples using hplc-fluorescence method
dc.typeThesis
dc.description.degreeMS
dc.description.departmentEnvironmental Health Science
dc.description.majorEnvironmental Health
dc.description.advisorJia-Sheng Wang
dc.description.committeeJia-Sheng Wang
dc.description.committeeXiaozhong Yu
dc.description.committeeLili Tang


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