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dc.contributor.authorDeng, Zhaojie
dc.description.abstractSingle-cell analysis has become crucial for uncovering the underlying mechanism for cell heterogeneity. Different biological questions pose different challenges for single-cell analysis. In order to answer questions like, whether a single-cell has a biological clock and how the clocks synchronize among cells to overcome the heterogeneity, continuous long-term measurement on large numbers of single-cells is required. However traditional measurement techniques usually involve measurement on millions of cells. My dissertation addresses these challenges by developing a microfluidic droplet platform capable of measuring the biological clock on >1000 Neurospora crassa single-cells for up to 10 days. The results show that in Neurospora crassa a single cell has the three major properties of a biological clock: a circadian oscillator, light entertainment, and temperature compensation and that single-cells synchronize their biological clock with each other possibly through quorum sensing.
dc.rightsOn Campus Only Until 2019-12-01
dc.subjectSingle-cell analysis
dc.subjectMicrofluidic droplets
dc.subjectNeurospora crassa
dc.subjectCircadian rhythm
dc.subjectBiological clock
dc.subjectStochastic noise
dc.titleSingle-cell analysis on the biological clock using microfluidic droplets
dc.description.majorBiological and Agricultural Engineering
dc.description.advisorLEIDONG MAO
dc.description.advisorJonathan Arnold
dc.description.committeeLEIDONG MAO
dc.description.committeeJonathan Arnold
dc.description.committeeXianqiao Wang
dc.description.committeeHeinz-Bernd Schuttler

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