Show simple item record

dc.contributor.authorDeng, Zhaojie
dc.date.accessioned2018-04-10T04:30:16Z
dc.date.available2018-04-10T04:30:16Z
dc.date.issued2017-12
dc.identifier.otherdeng_zhaojie_201712_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/deng_zhaojie_201712_phd
dc.identifier.urihttp://hdl.handle.net/10724/37686
dc.description.abstractSingle-cell analysis has become crucial for uncovering the underlying mechanism for cell heterogeneity. Different biological questions pose different challenges for single-cell analysis. In order to answer questions like, whether a single-cell has a biological clock and how the clocks synchronize among cells to overcome the heterogeneity, continuous long-term measurement on large numbers of single-cells is required. However traditional measurement techniques usually involve measurement on millions of cells. My dissertation addresses these challenges by developing a microfluidic droplet platform capable of measuring the biological clock on >1000 Neurospora crassa single-cells for up to 10 days. The results show that in Neurospora crassa a single cell has the three major properties of a biological clock: a circadian oscillator, light entertainment, and temperature compensation and that single-cells synchronize their biological clock with each other possibly through quorum sensing.
dc.languageeng
dc.publisheruga
dc.rightsOn Campus Only Until 2019-12-01
dc.subjectSingle-cell analysis
dc.subjectMicrofluidic droplets
dc.subjectNeurospora crassa
dc.subjectCircadian rhythm
dc.subjectBiological clock
dc.subjectStochastic noise
dc.subjectSynchronization
dc.titleSingle-cell analysis on the biological clock using microfluidic droplets
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentEngineering
dc.description.majorBiological and Agricultural Engineering
dc.description.advisorLEIDONG MAO
dc.description.advisorJonathan Arnold
dc.description.committeeLEIDONG MAO
dc.description.committeeJonathan Arnold
dc.description.committeeXianqiao Wang
dc.description.committeeHeinz-Bernd Schuttler


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record