Discovery, characterization, and utilization of Rpp genes for resistance against soybean rust
Childs, Silas Paul
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Soybean rust is a disease of soybean caused by Phakopsora pachyrhizi Syd. & P. Syd and Rpp genes can help protect soybean from this disease. In this study, a novel Rpp gene was mapped to a 154 kb interval on chromosome 19 in PI 605823 and named Rpp7. In addition, five germplasm accessions were identified which had unique reaction phenotypes to nine diverse P. pachyrhizi isolates and may carry novel Rpp alleles. Four new sources of resistance were mapped to either the Rpp3 or Rpp6 locus using bulked segregant analysis. Eleven accessions with a putatively novel Rpp3 allele were identified with a KASP™ marker developed within an Rpp3 candidate gene region. In addition, 11 breeding lines with stacked Rpp1 and Rpp3 loci were selected with molecular markers, and the introgression sizes of Rpp loci were estimated in 13 breeding lines or NILs using the SoySNP50K iSelect Beadchips.