Evaluation and application of enteric virus recovery techniques for environmental water monitoring
Phillips, Annie Rebecca
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Virus recovery techniques must preserve enteric viruses present in environmental waters and remove co-concentrated PCR inhibitory compounds. The aim of this study was to evaluate and apply virus recovery techniques for use in environmental water monitoring. For each technique, the percent recovery was determined for multiple enteric viral pathogens and process control viruses. Poly-ethylene glycol precipitation was shown to be the main source of viral loss, with percent recoveries ranging from 0.0% to 4.7%. Chloroform extraction and ultrafiltration had percent recoveries ranging from 277.2% to 0.6% and 62.5% to 9.7% respectively. The ultrafiltration technique was the most effective at removing PCR inhibitory compounds. Stream samples from Gwinnett County, Georgia were concentrated using the combined methods to test for multiple human enteric viral pathogens, with one sample testing positive for Sapovirus. This study highlights the need for more efficient recovery techniques to avoid underestimating possible public health risks.