Surgical models of renal interstitial fibrosis in cats
Dickerson, Vanna Marie
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Surgical renal ischemia (RI) has been extensively studied in other species for its utility in modeling kidney disease. The purpose of this work was to develop a surgical RI protocol which produced sufficient and consistent renal injury and fibrosis to be utilized as a model of acute kidney injury and/or chronic kidney disease in cats, with acceptable morbidity. We evaluated 15 and 30 minute bilateral RI, and 60 and 90 minute unilateral RI with a delayed contralateral nephrectomy. Serum creatinine, urine specific gravity, and plasma clearance of iohexol were assessed at several time points. Descriptive and objective histopathology measures were performed. Neither bilateral RI duration provided sufficient renal injury, and injury seen following 60 minute unilateral RI was inconsistent. Ninety minute unilateral RI produced consistent renal injury and fibrosis, however the mortality rate following nephrectomy was unacceptable. Future models should incorporate 90 minute unilateral RI without a contralateral nephrectomy.