Show simple item record

dc.contributor.authorMoore, Christina Ann
dc.date.accessioned2016-11-22T05:30:10Z
dc.date.available2016-11-22T05:30:10Z
dc.date.issued2016-05
dc.identifier.othermoore_christina_a_201605_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/moore_christina_a_201605_ms
dc.identifier.urihttp://hdl.handle.net/10724/36273
dc.description.abstractToxoplasma gondii is an obligate intracellular parasite that can infect any nucleated cell. The parasite invades host cells, replicates inside a parasitophorous vacuole and egresses to invade more host cells. This lytic cycle is associated with the tissue damage that accompanies the acute infection and is linked to the pathology of toxoplasmosis. Fluctuations of cytoplasmic Ca2+ are linked to the activation of the cellular processes involved in each step of the lytic cycle. However, the mechanism by which cytoplasmic Ca2+ is stimulated preceding egress is unknown. Using genetic Ca2+ indicators expressed in the cytosol of the parasites and also in the host cell, we show that cytoplasmic Ca2+ increases in the intracellular parasites prior to both artificially stimulated and natural egress. We demonstrated that extracellular Ca2+ entry enhances the events surrounding egress and that specific Ca2+ peaks observed prior to egress could correspond to signaling pathways regulating this step.
dc.languageeng
dc.publisheruga
dc.rightsOn Campus Only Until 2018-05-01
dc.subjectToxoplasma gondii (T. gondii)
dc.subjectCalcium signaling
dc.subjectGenetically-encoded calcium indicators
dc.subjectLytic cycle
dc.subjectEgress
dc.titleThe role of Ca2+ influx during Toxoplasma gondii egress from host cells
dc.typeThesis
dc.description.degreeMS
dc.description.departmentCellular Biology
dc.description.majorCellular Biology
dc.description.advisorSilvia Moreno
dc.description.committeeSilvia Moreno
dc.description.committeeVasant Muralidharan
dc.description.committeeJames D. Lauderdale


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record