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dc.contributor.authorParvathaneni, Rajiv Krishna
dc.description.abstractPearl millet is one of the most important subsistence crops grown in India and sub-Saharan Africa. In many cereal crops, reduced height is a key trait for enhancing yield and dwarf mutants have been extensively used in breeding to reduce yield loss due to lodging under intense management. In pearl millet, the recessive d2 dwarfing gene has been deployed widely in commercial germplasm grown in India, the US and Australia. Despite its importance, very little research has gone into determining the identity of the d2 gene. We used comparative information, genetic mapping in two F2 populations representing a total of some 1500 progeny, and haplotype analysis of three tall and three dwarf inbred lines to delineate the d2 region by two genetic markers that, in sorghum, define a region of 410 kb with 40 annotated genes. One of the sorghum genes annotated within this region is ABCB1 which encodes a P-glycoprotein involved in auxin transport. This gene had previously been shown to underlie the economically important dw3 dwarf mutation in sorghum. The cosegregation of ABCB1 with the d2 phenotype, its differential expression in the tall inbred ICMP 451 and the dwarf inbred Tift 23DB, and the similar phenotype of stacked lower internodes in the sorghum dw3 and pearl millet d2 mutants suggest that ABCB1 is a likely candidate for d2.
dc.rightsOn Campus Only Until 2017-12-01
dc.subjectcomparative genomics
dc.subjecthaplotype analysis
dc.titleIsolation, characterization and the dynamic structure of the pearl millet d2 dwarfing gene
dc.description.departmentCrop and Soil Sciences
dc.description.majorPlant Breeding, Genetics and Genomics
dc.description.advisorKatrien Devos
dc.description.committeeKatrien Devos
dc.description.committeeXiaoyu Zhang
dc.description.committeePeggy Ozias-Akins
dc.description.committeeRichard Meagher
dc.description.committeeJohn Burke

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