Enhanced, selective MRS medium and RT-PCR for detection of Lactobacillus animalis added to cattle feed
Johnson, Kasey Kristin
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Probiotics provide enormous potential for preventing and treating infectious diseases as an alternative to antimicrobials. It therefore has significant place in the market place. A challenge is the ability to quality control probiotics. This is important in the case of nutritional supplements and additives for animal feed. In order to correlate effects, there is a need to be able to monitor the viability and abundance of the organism(s) in the commercial product. This research focused on modification of deMann, Rogosa and Sharpe (MRS) medium to enhance its selectivity for isolating the probiotic Lactobacillus animalis LA51 added to cattle feed. An enrichment method utilizing modified MRS medium with enhanced selectivity for this strain of Lactobacillus was developed for detection of viable L. animalis LA51 in feed. In addition, a real time PCR was applied towards the specific detection of L. animalis LA51 against a background of other lactic acid bacteria.