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dc.contributor.authorYang, Daoyuan
dc.date.accessioned2016-03-18T04:30:17Z
dc.date.available2016-03-18T04:30:17Z
dc.date.issued2015-08
dc.identifier.otheryang_daoyuan_201508_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/yang_daoyuan_201508_ms
dc.identifier.urihttp://hdl.handle.net/10724/34771
dc.description.abstractA flow system was built to monitor biofilm growth on the inner surface of stainless steel tubes in a wide range of time intervals with adjustable initial microbial populations. Biofilm formation was detected by total plate counting (TPC) and electrochemical impedance spectroscopy (EIS) methods. The system was tested using Pseudomonas putida biofilms. Bacteria suspensions were flowing through the system continuously and maintained at 5.1 +/- 0.3 log CFU/ml for the entire duration of the experiment period. Biofilm was detected after 16 h by TPC, while impedance started to decrease at 28 h when biofilm populations were greater than 2.3 × 105 CFU/swab. The regression equation for the corrected Zreal (Zrc) versus log biofilm colony counts (C, 5.2 < C < 7.0) was Zrc = -23.3 C + 100.9 with R2 = 0.772.
dc.languageeng
dc.publisheruga
dc.rightsOn Campus Only Until 2017-08-01
dc.subjectBiofilm
dc.subjectFlow system
dc.subjectElectrochemical impedance spectroscopy
dc.titleDesign and construction of a flow system for controlled biofilm growth and electrochemical monitoring
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFood Science and Technology
dc.description.majorFood Science
dc.description.advisorJosé I. Reyes De Corcuera
dc.description.committeeJosé I. Reyes De Corcuera
dc.description.committeeRonald B. Pegg
dc.description.committeeMark Harrison


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