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dc.contributor.authorMostafa, Mahdi
dc.contributor.authorSiadat, Seyed D
dc.contributor.authorShahcheraghi, Fereshteh
dc.contributor.authorVaziri, Farzam
dc.contributor.authorJaponi-Nejad, Alireza
dc.contributor.authorVand Yousefi, Jalil
dc.contributor.authorRajaei, Bahareh
dc.contributor.authorHarifi Mood, Elnaz
dc.contributor.authorEbrahim zadeh, Nayyereh
dc.contributor.authorMoshiri, Arfa
dc.contributor.authorSeyed Siamdoust, Seyed A
dc.contributor.authorRahbar, Mohamad
dc.date.accessioned2015-09-01T18:28:38Z
dc.date.available2015-09-01T18:28:38Z
dc.date.issued2015-07-31
dc.identifier.citationBMC Microbiology. 2015 Jul 31;15(1):152
dc.identifier.urihttp://dx.doi.org/10.1186/s12866-015-0488-3
dc.identifier.urihttp://hdl.handle.net/10724/32029
dc.description.abstractAbstract Background To investigate antibiotic resistance, the occurrence and distribution of class 1 and 2 integrons in multidrug- resistant Staphylococcus aureus isolates from hospitals in Tehran, Iran. The isolates were examined for susceptibility to antimicrobial agents. The mecA gene, class 1 and 2 integrons were detected by PCR. Integrase positive strains were further analysed for the presence of resistance gene cassettes using specific primers and were sequenced. Results Among 139 S.aureus isolates, 109 (78.4 %) and 112 (80.5 %) strains were considered as multidrug resistant and mecA positive, respectively. Class 1 integrons and internal variable regions were found in 72.6 % (101/139) and 97 % (98/101) and class 2 integrons and variable regions also in 35.2 % (49/139) and 65.3 % (32/49) of S.aureus clinical isolates, respectively. Twelve distinct cassette arrays were found, containing genes encoding resistance to β-lactams, aminoglycosides, streptothricin, trimethoprim, chloramphenicol,a putative glucose dehydrogenase precursor and a protein with unknown function. Gene cassette arrays aadB, aadA2 and dhfrA1-sat2-aadA1 were common in S.aureus isolates. We detected a completely new gene cassettes which contained aadB, oxa2, aacA4, orfD-aacA4-catB8, aadB-catB3, orfD-aacA4 and aadB-aadA1-cmlA6 of class 1 and dhfrA1-sat2-aadA1, dhfrA11, dhfrA1-sat2 of class 2 integrons. Conclusions This is the first study to report carriage of class 1 and 2 integrons and associated gene cassettes among in S.aureus isolates from Iran.
dc.titleVariability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran
dc.typeJournal Article
dc.date.updated2015-07-31T03:42:25Z
dc.language.rfc3066en
dc.rights.holderMostafa et al.


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