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dc.contributor.authorTakeyama, Masayuki
dc.contributor.authorYoneda, Masahiko
dc.contributor.authorGosho, Masahiko
dc.contributor.authorIwaki, Masayoshi
dc.contributor.authorZako, Masahiro
dc.date.accessioned2015-09-01T18:06:28Z
dc.date.available2015-09-01T18:06:28Z
dc.date.issued2015-07-30
dc.identifier.citationBiological Research. 2015 Jul 30;48(1):42
dc.identifier.urihttp://dx.doi.org/10.1186/s40659-015-0034-7
dc.identifier.urihttp://hdl.handle.net/10724/31943
dc.description.abstractAbstract Background Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogenesis in retinal pigment epithelium (RPE). Results We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium–derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased. Conclusions RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.
dc.titleDecreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
dc.typeJournal Article
dc.date.updated2015-07-29T19:00:57Z
dc.language.rfc3066en
dc.rights.holderTakeyama et al.


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