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    Molecular characterization of systemic sclerosis esophageal pathology identifies inflammatory and proliferative signatures

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    13075_2015_Article_695.pdf (3.536Mb)
    Date
    2015-07-29
    Author
    Taroni, Jaclyn N
    Martyanov, Viktor
    Huang, Chiang-Ching
    Mahoney, J. M
    Hirano, Ikuo
    Shetuni, Brandon
    Yang, Guang-Yu
    Brenner, Darren
    Jung, Barbara
    Wood, Tammara A
    Bhattacharyya, Swati
    Almagor, Orit
    Lee, Jungwha
    Sirajuddin, Arlene
    Varga, John
    Chang, Rowland W
    Whitfield, Michael L
    Hinchcliff, Monique
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    Abstract
    Abstract Introduction Esophageal involvement in patients with systemic sclerosis (SSc) is common, but tissue-specific pathological mechanisms are poorly understood. There are no animal scleroderma esophagus models and esophageal smooth muscle cells dedifferentiate in culture prohibiting in vitro studies. Esophageal fibrosis is thought to disrupt smooth muscle function and lead to esophageal dilatation, but autopsy studies demonstrate esophageal smooth muscle atrophy and the absence of fibrosis in the majority of SSc cases. Herein, we perform a detailed characterization of SSc esophageal histopathology and molecular signatures at the level of gene expression. Methods Esophageal biopsies were prospectively obtained during esophagogastroduodenoscopy in 16 consecutive SSc patients and 7 subjects without SSc. Upper and lower esophageal biopsies were evaluated for histopathology and gene expression. Results Individual patient’s upper and lower esophageal biopsies showed nearly identical patterns of gene expression. Similar to skin, inflammatory and proliferative gene expression signatures were identified suggesting that molecular subsets are a universal feature of SSc end-target organ pathology. The inflammatory signature was present in biopsies without high numbers of infiltrating lymphocytes. Molecular classification of esophageal biopsies was independent of SSc skin subtype, serum autoantibodies and esophagitis. Conclusions Proliferative and inflammatory molecular gene expression subsets in tissues from patients with SSc may be a conserved, reproducible component of SSc pathogenesis. The inflammatory signature is observed in biopsies that lack large inflammatory infiltrates suggesting that immune activation is a major driver of SSc esophageal pathogenesis.
    URI
    http://dx.doi.org/10.1186/s13075-015-0695-1
    http://hdl.handle.net/10724/31840
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