Show simple item record

dc.contributor.authorZhan, Yueping
dc.contributor.authorXiang, Fenfen
dc.contributor.authorWu, Rong
dc.contributor.authorXu, Jian
dc.contributor.authorNi, Zhenhua
dc.contributor.authorJiang, Jiemin
dc.contributor.authorKang, Xiangdong
dc.date.accessioned2015-09-01T17:16:29Z
dc.date.available2015-09-01T17:16:29Z
dc.date.issued2015-07-30
dc.identifier.citationJournal of Ovarian Research. 2015 Jul 30;8(1):48
dc.identifier.urihttp://dx.doi.org/10.1186/s13048-015-0178-7
dc.identifier.urihttp://hdl.handle.net/10724/31763
dc.description.abstractAbstract Background The low effectiveness of anticancer drugs remains a major unresolved obstacle to successful chemotherapy. Recently, much evidence on the roles of miRNAs in determining drug-sensitivity/resistance has been emerging. The relationship between miRNA-149 expression and paclitaxel chemoresistance in human ovarian cancer cells remains largely unknown. Methods This study investigated the relationship between miRNA-149 expression and the sensitivity of ovarian cancer A2780 cells to paclitaxel treatment. To achieve the down-regulation of miRNA-149 gene expression in A2780 cell line, the cells were infected with lentivirus carrying inhibitor of miRNA-149. Western blot and qRT-PCR were used to detect relevant protein levels and the expressions of mRNAs of interest. Cell proliferation was measured by CCK-8 assay. Flow cytometry was used to measure cell cycle and apoptosis. Transwell migration assay was used to observe the change of migration of transfected cells. Results Down-regulation of miRNA-149 decreased the sensitivity of ovarian cancer A2780 cells to paclitaxel. After paclitaxel treatment, decreased apoptosis and G2 phase ratio, increased cell migration, increased level of Bcl-2, and decreased level of Bax were found in miRNA-149-down-regulated A2780 cells. MiRNA-149 down-regulation resulted in increased expression of MyD88 in A2780 cells. Down-regulation of miRNA-149 in A2780 cells increased MyD88 expression and decreased their sensitivity to paclitaxel treatment. Conclusion Our findings suggest that miRNA-149 mediates the susceptibility of paclitaxel by regulating MyD88 expression in ovarian cancer cells.
dc.titleMiRNA-149 modulates chemosensitivity of ovarian cancer A2780 cells to paclitaxel by targeting MyD88
dc.typeJournal Article
dc.date.updated2015-07-29T18:26:24Z
dc.language.rfc3066en
dc.rights.holderZhan et al.


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record