Show simple item record

dc.contributor.authorMariani, Erminia
dc.contributor.authorCanella, Valentina
dc.contributor.authorBerlingeri, Andrea
dc.contributor.authorBielli, Alessandra
dc.contributor.authorCattini, Luca
dc.contributor.authorLandini, Maria P
dc.contributor.authorKon, Elizaveta
dc.contributor.authorMarcacci, Maurilio
dc.contributor.authorDi Matteo, Berardo
dc.contributor.authorFilardo, Giuseppe
dc.date.accessioned2015-09-01T16:54:45Z
dc.date.available2015-09-01T16:54:45Z
dc.date.issued2015-07-30
dc.identifier.citationBMC Microbiology. 2015 Jul 30;15(1):149
dc.identifier.urihttp://dx.doi.org/10.1186/s12866-015-0482-9
dc.identifier.urihttp://hdl.handle.net/10724/31678
dc.description.abstractAbstract Background Human platelets are a rich reservoir of molecules that promote regenerative processes and microbicidal activity. This activity might be increased by concentration in platelet-rich plasma (PRP) products and modulated by the presence of leukocytes. Despite extensive use in clinical procedures, only few studies have investigated PRP’s real microbicidal potential. Therefore, this study aimed at comparing the in vitro microbicidal activity of platelets and leukocyte-enriched PRP (L-PRP) to pure platelet-rich plasma (P-PRP) and the contribution of leukocytes to microbicidal properties. Antimicrobial effects of P- and L-PRP were tested against Escherichia Coli, Staphylococcus Aureus, Klebsiella Pneumoniae, Pseudomonas Aeruginosa and Enterococcus Faecalis. Furthermore, L-PRP was frozen (L-PRP cryo) to assess whether the preparation maintained in vitro characteristics. Microbicidal proteins released by the three preparations were also evaluated. Results L-PRP, L-PRP cryo and P-PRP generally induced comparable bacterial growth inhibition for up to 4 h’ incubation, range 1–4 log. MIP-1α, RANTES, GRO-α, IL-8, NAP-2, SDF-1α and IL-6 showed strong microbicidal potential. Conclusions We found in vitro antibacterial activity of L-PRP and P-PRP and the possibility to cryopreserve L-PRP, without important changes to its effectiveness; similar microbicidal activity between preparations containing or not leukocytes; and the contribution of three new molecules (NAP-2, SDF-1α and IL-6).
dc.titleLeukocyte presence does not increase microbicidal activity of Platelet-rich Plasma in vitro
dc.typeJournal Article
dc.date.updated2015-07-29T18:12:07Z
dc.language.rfc3066en
dc.rights.holderMariani et al.


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record