Show simple item record

dc.contributor.authorWang, Zhao
dc.contributor.authorWu, Ningbin
dc.contributor.authorTesfaye, Abeba
dc.contributor.authorFeinstone, Stephen
dc.contributor.authorKumar, Ajit
dc.date.accessioned2015-08-31T13:27:21Z
dc.date.available2015-08-31T13:27:21Z
dc.date.issued2015-07-27
dc.identifier.citationInfectious Agents and Cancer. 2015 Jul 27;10(1):24
dc.identifier.urihttp://dx.doi.org/10.1186/s13027-015-0018-9
dc.identifier.urihttp://hdl.handle.net/10724/31580
dc.description.abstractAbstract Background and Aims Hepatitis C virus (HCV) infection is a major risk factor for chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Our aim is to explore molecular changes that underlie HCV infection-associated HCC in a humanized mouse model, in order to identify markers of HCC progression. Methods Liver proteins from human hepatocyte-engrafted and HCV-infected MUP-uPA/SCID/Bg mice were compared with either uninfected controls or HCV-infected but HCC-negative mice by Western blotting. MicroRNA markers of HCC positive or uninfected mouse liver were analyzed by RT-PCR. Results We describe the depletion of tumor suppressor proteins and induction of oncoproteins and oncogenic microRNAs (oncomiRs) in HCV-infection associated HCC. Similar depletion of PTEN protein in both HCC-positive and HCV-infected but HCC-negative liver suggests that PTEN depletion is an early, precancerous marker of HCC. By contrast, induction of oncoprotein cMyc, oncomiRs (miR21, miR221 and miR141) and inflammatory response proteins correspond to HCC progression. Conclusions While the loss of PTEN is important for the initiation of HCV infection-associated HCC, PTEN depletion by itself is insufficient for tumor progression. Liver tumor progression requires induction of oncoproteins and oncomiRs. Overall, human hepatocyte-engrafted (MUP-uPA/SCID/Bg) mice provide a suitable small animal model for studying the effects of oncogenic changes that promote HCV infection associated HCC.
dc.titleHCV infection-associated hepatocellular carcinoma in humanized mice
dc.typeJournal Article
dc.date.updated2015-07-29T17:53:38Z
dc.language.rfc3066en
dc.rights.holderWang et al.


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record