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dc.contributor.authorYang, Yiwen
dc.description.abstractThe Arabidopsis gene, PARVUS/AtGATL1, belongs to the Galacturonosyl-transferase-Like (GATL) gene family, which is part of CAZy Family GT8. Five GATL sequences of the moss Physcomitrella patens (PpGATL-A,B,C,D,E) have been identified through phylogenetic analyses. The five PpGATLs form a sister group that lies basal to all of the vascular plant GATLs. Homozygous parvus/gatl1 mutants in Arabidopsis thaliana are semi-sterile and exhibit a dwarf growth phenotype with collapsed xylem. Cell walls of parvus/gatl1 show significant reductions in xylose content compared with wild-type cell walls. Constitutive expression of PpGATL-A in the Arabidopsis parvus mutant rescues the defects conferred by the parvus mutation, including changes in plant growth and tissue morphology, collapsed xylem, decreased xylose content based on cell wall monosaccharide composition and the altered overall cell wall structure as indicated by glycome profiling. These findings suggest that PpGATL-A is a functional ortholog of PARVUS/GATL1 and at least one basic function of GATL1 is conserved in highly divergent land plants. Sub-cellular localization experiments showed that PpGATL-A is a Golgi-localized protein, as is the Arabidopsis AtGATL1. Promoter
dc.rightsOn Campus Only Until 2016-12-01
dc.subjectPhyscomitrella patens
dc.subjectplant cell walls
dc.subjectleafy gametophores
dc.subjecthomologous recombination
dc.subjecttargeted gene knockout
dc.titleA functional ortholog of the arabidopsis parvus/atgatl1 gene affects growth and development in the moss Physcomitrella patens
dc.description.departmentPlant Biology
dc.description.majorPlant Biology
dc.description.advisorMichael Hahn
dc.description.committeeMichael Hahn
dc.description.committeeDebra Mohnen
dc.description.committeeJames Leebens-Mack
dc.description.committeeR. Kelly Dawe
dc.description.committeeAlan Darvill

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