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dc.contributor.authorShane, Hillary Lee
dc.date.accessioned2015-08-05T04:30:12Z
dc.date.available2015-08-05T04:30:12Z
dc.date.issued2014-12
dc.identifier.othershane_hillary_l_201412_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/shane_hillary_l_201412_phd
dc.identifier.urihttp://hdl.handle.net/10724/31509
dc.description.abstractMucosal surfaces represent the major portal by which pathogens enter the body, yet there is limited understanding of how CD8+ T cell responses develop and are maintained at these sites. The majority of knowledge on CD8+ T cell responses and memory formation has been amassed in models of acute, systemic infections, even though it is appreciated that mucosal sites consist of immunological environments unique from sites in which responses to systemic infections develop. Moreover, a firm understanding of how memory is generated in mucosal sites is important for the development of vaccines, which may employ a systemic or mucosal route of immunization, such as those directed against influenza virus. Vaccines that target protective CD8+ T cell responses are of particular interest for influenza, as memory CD8+ T cells can limit severe disease and can offer protection against multiple influenza subtypes. This study shows that the respiratory environment can directly influence CD8+ cell responses, resulting in localized changes in CD8+ T cell memory formation as well as broadly inhibiting the formation of long-lived memory cells. We show that the mucosal cytokine thymic stromal lymphopoietin (TSLP) is produced early following mucosal influenza infection, and acts on CD8+ effector cells in a direct and non-redundant way, promoting the proliferation of these cells at the site of infection. This early response influences memory development resulting in more of an effector memory population of cells. I go on to show that by comparing vesicular stomatitis virus infection delivered by the intranasal or intravenous route, that the respiratory environment results in memory CD8+ T cell population that is skewed from the archetypical memory developmental programs defined in systemic models of infection, resulting in numerically deficient memory. Together this work suggests that the respiratory environment can uniquely transform CD8+ T responses towards a more short-lived population of cells, and that protective vaccination strategy will require thoughtful modifications to bypass the restrictions conferred by the respiratory environment and promote memory development.
dc.languageeng
dc.publisheruga
dc.rightsOn Campus Only Until 2016-12-01
dc.subjectCD8+ T cells
dc.subjectMemory
dc.subjectCytokines
dc.subjectRespiratory
dc.subjectInfluenza
dc.titleDevelopment of anti-viral CD8+ T cell memory in the respiratory environment
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentCellular Biology
dc.description.majorCellular Biology
dc.description.advisorKimberly Klonowski
dc.description.committeeKimberly Klonowski
dc.description.committeeRick Tarleton
dc.description.committeeDonald Harn
dc.description.committeeDaniel G. Colley
dc.description.committeeDan Campbell


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