A molecular investigation of the effects of the loss of linker histone H1 in the filamentous fungus Neurospora crassa
Seymour, Michael Justin
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This study represents an initial investigation into the effects on chromatin structure and gene regulation of N. crassa following the deletion of the gene for the linker histone H1 (hho). This study concentrated on the use of high throughput sequencing techniques and the analysis of the resulting large data sets generated via mRNA-seq, ChIP-seq, and MNase-seq. Though no differential patterns of H1 occupancy were found, significant changes in the transcription rate of a small set of genes in the H1 deletion (Δhho) strain with accompanying changes in nucleosome stability in the NFR region of these genes indicates a role for H1 in gene regulation and the maintenance of chromatin structure in this organism.