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dc.contributor.authorMihala, Alexandra Gabriela
dc.date.accessioned2014-10-09T04:30:27Z
dc.date.available2014-10-09T04:30:27Z
dc.date.issued2014-05
dc.identifier.othermihala_alexandra_g_201405_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/mihala_alexandra_g_201405_phd
dc.identifier.urihttp://hdl.handle.net/10724/30547
dc.description.abstractKey developmental processes in embryogenesis are modulated by transcription factors and signaling molecules whose spatio-temporal expression patterns need to be tightly regulated by cis-regulatory elements or enhancers. I first set out to investigate enhancer redundancy in mice using the Tbx4 locus. I show that the two hindlimb specific regulatory elements of Tbx4, HLEA and HLEB, although have overlapping expression patterns in this tissue, do serve distinct roles and are therefore not redundant. Further investigation of the active appendage regulome has uncovered a large overlap between enhancers active in limbs and those active in the genital tubercle. One of these regulatory elements is HLEB which, besides gene expression in the hindlimbs, also drives robust expression in the genital tubercle. I show that this enhancer is not only active in two distinct appendage types, but also is functionally important for the proper development of both tissues.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectEnhancer
dc.subjectCis-regulatory element
dc.subjectHindlimb
dc.subjectAppendage
dc.subjectGenital tubercle
dc.subjectTbx4
dc.subjectHLEA
dc.subjectHLEB
dc.titleStudying enhancer function using the Tbx4 locus as a model
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentGenetics
dc.description.majorGenetics
dc.description.advisorDoug Menke
dc.description.committeeDoug Menke
dc.description.committeeNancy R. Manley
dc.description.committeeJames Lauderdale
dc.description.committeeBrian Condie
dc.description.committeeHaini Cai


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