Show simple item record

dc.contributor.authorLuis, Jane Marian
dc.date.accessioned2014-10-03T04:30:20Z
dc.date.available2014-10-03T04:30:20Z
dc.date.issued2014-05
dc.identifier.otherluis_jane-marian_201405_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/luis_jane-marian_201405_ms
dc.identifier.urihttp://hdl.handle.net/10724/30527
dc.description.abstractAflatoxin is a major concern in peanut production especially in areas experiencing drought and high temperatures. The best way to manage aflatoxin contamination would be to integrate host resistance, identification of causal organism, and develop improved management strategies. Phenotyping of seven peanut genotypes identified Tifguard and Tifrunner as having better drought-coping ability than the other genotypes. Aspergillus section Flavi isolates collected from different geographical locations were identified through morphological and genetic variation. Evaluation of the effect of sample size in aflatoxin extraction demonstrated that the standard subsampling of 300 g into 100 g can be reduced to subsampling 100 g into 25 g. Immunochromatographic test strips were confirmed to have comparable aflatoxin detection results with fluorometry method and can be used under continuous high or fluctuating temperatures. Also, a real-time PCR (qPCR) assay using species-specific primers targeting the aflS gene effectively detected A. flavus and/or A. parasiticus in peanut seeds.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectAflatoxin
dc.subjectpeanut
dc.subjectdrought-tolerance
dc.subjectAspergillus flavus
dc.subjectA. parasiticus
dc.subjectsample size
dc.subjectimmunochromatographic test strips
dc.subjectreal-time PCR
dc.titleManagement of aflatoxin through drought stress phenotyping, Aspergillus section Flavi characterization and accurate quantification of Aspergillus and aflatoxin contamination
dc.typeThesis
dc.description.degreeMS
dc.description.departmentPlant Pathology
dc.description.majorPlant Pathology
dc.description.advisorAnthony Glenn
dc.description.advisorRobert Kemerait, Jr.
dc.description.committeeAnthony Glenn
dc.description.committeeRobert Kemerait, Jr.
dc.description.committeePeggy Ozias-Akins
dc.description.committeeCorley Holbrook


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record