The effect of delayed serum separation and storage temperature on the rate of serum glucose concentration decline in alpacas, horses, dogs, and sturgeon
Collicutt, Nancy Brown
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The objective was to investigate the effect of storage parameters on serum glucose in blood samples from four species. Blood specimens were divided into 7 no-additive tubes and serum separated from one sample after one hour (reference sample). Remaining samples were stored at 4°C and 25°C, then centrifuged and serum glucose measured at 2, 4, and 8 hours post-collection. Serum glucose declines for all samples stored at 4°C were not significantly different, except for the 8 hour samples from sturgeon and dogs. At 25°C, serum glucose was comparable to reference values at 2 hours in the horse, sturgeon, and alpaca, but significantly lower at 4 and 8 hours in those species and at all time points in dogs. Storage at 4°C limits serum glucose decline for at least 4 hours in all species tested. At 25°C, serum-clot contact time should not exceed 1 hour in dogs and 2 hours in horses, alpacas, and sturgeon.