Show simple item record

dc.contributor.authorVagasi, Alexandra Sylvia
dc.date.accessioned2014-07-04T04:30:19Z
dc.date.available2014-07-04T04:30:19Z
dc.date.issued2014-05
dc.identifier.othervagasi_alexandra_s_201405_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/vagasi_alexandra_s_201405_ms
dc.identifier.urihttp://hdl.handle.net/10724/30027
dc.description.abstractThis thesis discusses the creation of a cell culture system for germ cells from the nematode Caenorhabditis elegans. While a culture system exists for C. elegans embryonic cells, they have poor long term survival, and cannot be used for experiments after five days. As well, the media used for this embryonic culture leads to very rapid death of germ cells. Therefore, a better culture system is needed. Here, a method for harvesting germ cells is provided as well as a media, CeM1, that has been optimized so that these cultured germ cells can remain alive and at fairly constant numbers for at least 28 days. In addition, germ cell proliferation is shown for germ cells between days 1-4 in certain conditions, which is predicted to be a result of signals from bacteria.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectC. elegans
dc.subjectGermline
dc.subjectCell culture
dc.subjectGrowth media
dc.subjectCKI-2
dc.subjectGLP-1
dc.subjectDAF-16
dc.titleDefining the conditions for Caenorhabditis elegans germ cell primary cultures
dc.typeThesis
dc.description.degreeMS
dc.description.departmentCellular Biology
dc.description.majorCellular Biology
dc.description.advisorEdward Kipreos
dc.description.committeeEdward Kipreos
dc.description.committeeJacek Gaertig
dc.description.committeeHaini Cai


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record