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dc.contributor.authorLangley, Lauren Claire
dc.date.accessioned2014-06-24T04:30:17Z
dc.date.available2014-06-24T04:30:17Z
dc.date.issued2013-12
dc.identifier.otherlangley_lauren_c_201312_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/langley_lauren_c_201312_ms
dc.identifier.urihttp://hdl.handle.net/10724/29894
dc.description.abstractHuman norovirus is the primary cause of non-bacterial gastroenteritis world-wide. Porcine gastric mucins (PGM) contain histo-blood group antigens that are capable of binding multiple genotypes of HuNoV, unlike monoclonal antibodies (mAB) which are less cross-reactive. The purpose of this study was two-fold: 1) To determine the limit of detection (LOD) for PGM- and mAB- conjugated magnetic bead-based recovery assays in the context of elution buffers commonly used in Food Virology; 2) To determine if the addition PGM to existing low-cost recovery methods could increase virus recovery. When magnetic beads were coated with PGM, assay detection limits were lower than with mAB-coated beads for all buffer matrices tested. Neutral pH buffers were most compatible, providing a low LOD with minimal non-specific binding. Although repeatability was poor for the novel PGM assays, results warrant future exploration and are an important step in developing cost-effective, rapid and reliable method for norovirus detection.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectNorovirus
dc.subjectPorcine Gastric Mucins
dc.subjectSaliva
dc.subjectHisto-blood group antigens
dc.subjectMagnetic Beads
dc.subjectImmunomagnetic separation
dc.subjectNorwalk Virus
dc.titleNovel approaches to capturing and recovering human norovirus using porcine gastric mucins
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFood Science and Technology
dc.description.majorFood Science
dc.description.advisorJennifer Cannon
dc.description.committeeJennifer Cannon
dc.description.committeeYnes Ortega
dc.description.committeeJoseph Frank


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