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dc.contributor.authorBiswal, Ajaya K
dc.contributor.authorSoeno, Kazuo
dc.contributor.authorGandla, Madhavi L
dc.contributor.authorImmerzeel, Peter
dc.contributor.authorPattathil, Sivakumar
dc.contributor.authorLucenius, Jessica
dc.contributor.authorSerimaa, Ritva
dc.contributor.authorHahn, Michael G
dc.contributor.authorMoritz, Thomas
dc.contributor.authorJönsson, Leif J
dc.contributor.authorIsraelsson-Nordström, Maria
dc.contributor.authorMellerowicz, Ewa J
dc.date.accessioned2014-06-03T20:42:13Z
dc.date.available2014-06-03T20:42:13Z
dc.date.issued2014-01-22
dc.identifier.citationBiotechnology for Biofuels. 2014 Jan 22;7(1):11
dc.identifier.urihttp://dx.doi.org/10.1186/1754-6834-7-11
dc.identifier.urihttp://hdl.handle.net/10724/29788
dc.description.abstractAbstract Background Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. Results We show that aspen pectate lyase PL1-27, which degrades homogalacturonan and is expressed at the onset of secondary wall formation, can increase the solubility of wood matrix polysaccharides. Overexpression of this enzyme in aspen increased solubility of not only pectins but also xylans and other hemicelluloses, indicating that homogalacturonan limits the solubility of major wood cell wall components. Enzymatic saccharification of wood obtained from PL1-27-overexpressing trees gave higher yields of pentoses and hexoses than similar treatment of wood from wild-type trees, even after acid pretreatment. Conclusions Thus, the modification of pectins may constitute an important biotechnological target for improved wood processing despite their low abundance in woody biomass.
dc.titleAspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield
dc.typeJournal Article
dc.date.updated2014-04-02T10:59:13Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderAjaya K Biswal et al.; licensee BioMed Central Ltd.


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