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dc.contributor.authorAckerley, Jennifer Lynn
dc.date.accessioned2014-03-05T16:03:25Z
dc.date.available2014-03-05T16:03:25Z
dc.date.issued2002-08
dc.identifier.otherackerley_jennifer_l_200208_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/ackerley_jennifer_l_200208_ms
dc.identifier.urihttp://hdl.handle.net/10724/29473
dc.description.abstractClarification of orange juice is a quality defect attributed to pectinmethylesterase (PME). Thermolabile PMEs with different specific activities were added to stabilized juice. Particle size distribution, % transmittance, uronic acid analysis and SDS-PAGE were used to monitor clarification overtime. Juices with the highest specific activity clarified at the slowest rate and contained 36 and 13 kDa peptides. Juices with a 36 kDa and 27 kDa peptide clarified juices fastest. In fresh juice with cloud insoluble solids (PFJ) and juice with only cloud soluble solids (UCS), UCS formed a floc concurrently with clarification of PFJ. The clarification of juices adjusted to pH 7 was retarded due to electrostatic repulsion while juices at natural pH clarified more rapidly. These results show that serum-soluble factors, total charge and electrostatic interactions influence cloud destabilization.
dc.languageClarification of Valencia orange juice is influenced by specific activity of thermolabile pectinmethylesterase, inactive PME-pectin complexes and the changes in serum soluble components
dc.publisheruga
dc.rightspublic
dc.subjectPectinmethylesterase
dc.subjectPectin
dc.subjectCloud
dc.subjectOrange Juice
dc.subjectClarification
dc.subjectFloc
dc.subjectSpecific Activity
dc.subjectDegree of Esterification
dc.titleClarification of Valencia orange juice is influenced by specific activity of thermolabile pectinmethylesterase, inactive PME-pectin complexes and the changes in serum soluble components
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFood Science and Technology
dc.description.majorFood Science
dc.description.advisorLouise Wicker
dc.description.committeeLouise Wicker
dc.description.committeeMilena Corredig
dc.description.committeeWilliam Kerr


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