• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Fusion proteins engineered to characterize glycoprotein hormone-receptor interactions

    Thumbnail
    Date
    2002-08
    Author
    Schubert, Rebecca Lee
    Metadata
    Show full item record
    Abstract
    The family of glycoprotein hormones consists of four heterodimeric hormones: human chorionic gonadotropin, luteinizing hormone, thyroid stimulating hormone, and follicle stimulating hormone. Each hormone consists of a common a subunit and a hormonespecific b subunit, which together bind to the N-terminal extracellular domain of their G protein-coupled receptors, stimulating production of cAMP. Proper activation of these receptors is critical for control of normal reproduction and metabolism. Both hCG and LH bind the LH receptor, whereas TSH and FSH bind their unique receptors, TSHR and FSHR, respectively. Structure-function studies of homologous sequences within this family are hampered by the excessive amounts of hormone necessary to study noncognate or low affinity interactions. By fusing a hormone or part of a hormone to a receptor, weak binding events can be evaluated that would otherwise be undetectable. These methods revealed that hCG can activate the non-cognate receptors, TSHR and FSHR, in addition to its cognate receptor, LHR. The minimum number of residues of hCGb required to exhibit binding and activation activity can also be determined in this system. Low molecular weight hCG hormones, or mini-gonadotropins, retaining just one-third of the b subunit were sufficient to cause weak activation of LHR in the absence of full-length hCG. These studies provide valuable information concerning regions of specificity in hCG and the glycoprotein hormone receptors that are responsible for hormone binding and receptor activation, which will aid in the development of clinically relevant low molecular weight agonists and antagonists for these receptors.
    URI
    http://purl.galileo.usg.edu/uga_etd/schubert_rebecca_l_200208_phd
    http://hdl.handle.net/10724/29306
    Collections
    • University of Georgia Theses and Dissertations

    Related items

    Showing items related by title, author, creator and subject.

    • Potential functions of gonadotropin inhibitory hormone and dynorphin in broiler breeder reproduction 

      Stephens, Ashley Gera-Ann (uga, 2017-12)
      Broiler breeders given free access to feed can reach their ideal bodyweight for reproduction by 5 weeks of age, but functional sexual maturation of the brain does not occur until about 20 weeks of age. To match the ideal ...
    • In vivo consequences of the expression of constitutively active luteinizing hormone receptors 

      Meehan, Thomas Paul (uga, 2003-08)
      Luteinizing hormone (LH) is critical for reproduction in mammals. Its activity is delineated through the signaling of a member of the G-protein coupled receptor superfamily, the luteinizing hormone receptor (LHR), which ...
    • The molecular landscape of G protein signaling through the human luteinizing hormone receptor 

      DeMars, Geneva (uga, 2010-05)
      The molecular mechanism employed by the human luteinizing hormone receptor (hLHR), a G protein coupled receptor (GPCR) necessary for fertility and reproduction, to couple to its cognate signaling partner, the stimulatory ...

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback