Show simple item record

dc.contributor.authorLyon, Delina Yvonne-Marie
dc.date.accessioned2014-03-04T21:59:26Z
dc.date.available2014-03-04T21:59:26Z
dc.date.issued2002-08
dc.identifier.otherlyon_delina_y_200208_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/lyon_delina_y_200208_ms
dc.identifier.urihttp://hdl.handle.net/10724/29248
dc.description.abstractIn this research, two adjacent genes, shdC and shdD, were found necessary to encode an active 4-hydroxybenzoate decarboxylase (ShdCD) from Sedimentibacter hydroxybenzoicus JW/Z-1 (formerly Clostridium hydroxybenzoicum JW/Z-1). Homology searches of ShdCD revealed three homologues with the same genetic arrangement - the homologues to shdC and shdD adjacent to each other. These homologues are from Streptomyces sp.D7, Bacillus subtilis, and Escherichia coli O157:H7. The homologue from Streptomyces sp.D7 had already been identified as a vanillate decarboxylase in the laboratory of Dr. Julian Davies. The genes encoding the three homologues were cloned into E. coli JM109, expressed, and assayed for decarboxylase activity. The homologues from Streptomyces sp.D7 and B. subtilis are vanillate decarboxylases, and the one from E. coli O157:H7 is a 4-hydroxybenzoate decarboxylase. Based on sequences and enzyme activities, we propose that the decarboxylases from S. hydroxybenzoicus, B. subtilis, Streptomyces sp.D7, and E. coli O157:H7 represent a novel enzyme family.
dc.languageCharacterizing a proposed novel enzyme family of reversible hydroxyarylic acid decarboxylases
dc.publisheruga
dc.rightspublic
dc.subjectAromatic compound degradation
dc.subjectdecarboxylase
dc.subjectanaerobic
dc.subjectSedimentibacter hydroxybenzoicus
dc.subjecthydroxyarylic acid
dc.titleCharacterizing a proposed novel enzyme family of reversible hydroxyarylic acid decarboxylases
dc.typeThesis
dc.description.degreeMS
dc.description.departmentMicrobiology
dc.description.majorMicrobiology
dc.description.advisorJuergen Wiegel
dc.description.committeeJuergen Wiegel
dc.description.committeeEllen Neidle
dc.description.committeeTimothy Hoover


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record