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dc.contributor.authorJordan, Sarah Elizabeth
dc.date.accessioned2014-03-04T21:59:03Z
dc.date.available2014-03-04T21:59:03Z
dc.date.issued2002-08
dc.identifier.otherjordan_sarah_e_200208_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/jordan_sarah_e_200208_ms
dc.identifier.urihttp://hdl.handle.net/10724/29214
dc.description.abstractStreptomyces are filamentous, multi-cellular soil bacteria that undergo a complicated life cycle of differentation and secondary metabolite production. However, little is known about how these pathways function in the organizm. We sought to isolate new mutants in these processes in two species: S. coelicolor and S. venezuelae. This was accomplished through the use of intergeneric conjugation, in vitro transposition, and co-transformation. Through these methods we were able to identify 5 morphological mutants, 3 of which were caused by transposon insertion into a previously uncharacterized genes. Based on sequence analysis, putative functions of these gene products are: an integral membrane protein and tetR-family transcriptional regulator. We have not yet obtained sequence data for the third mutant gene.
dc.languageIsolation of morphological mutants in streptomyces species
dc.publisheruga
dc.rightspublic
dc.subjectStreptomyces
dc.subjectbld genes
dc.subjectIntergeneric conjuation
dc.subjectin vitro transposition
dc.subjectDifferentiation
dc.subjectAntibiotic production
dc.titleIsolation of morphological mutants in streptomyces species
dc.typeThesis
dc.description.degreeMS
dc.description.departmentGenetics
dc.description.majorGenetics
dc.description.advisorJanet Westpheling
dc.description.committeeJanet Westpheling
dc.description.committeeLarry Shimkets
dc.description.committeeAnna Karls


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