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dc.contributor.authorKrauss, Amy
dc.date.accessioned2014-03-04T21:13:11Z
dc.date.available2014-03-04T21:13:11Z
dc.date.issued2013-08
dc.identifier.otherkrauss_amy_201308_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/krauss_amy_201308_ms
dc.identifier.urihttp://hdl.handle.net/10724/29095
dc.description.abstractAnthocyanidins induce apoptosis in some cancer cell lines, but studies describing the dose-dependent effects on cell populations are limited. My objective was to evaluate the dose-dependent response of malvidin and delphinidin, anthocyanidins with different chemical structures, on cell viability and apoptosis in HT-29 cells compared to curcumin, a known inducer of apoptosis. Lower concentrations of anthocyanidins increased cell viability by 12-16% compared to control. Higher concentrations of ≥80 umol/L of curcumin, malvidin, and delphinidin decreased cell viability by 16-44%. Apoptosis was assessed by measuring caspase-3 and caspase-8 activities, as well as mitochondrial permeability. Only high concentrations (80 umol/L) of anthocyanidins and curcumin increased caspase-3 and caspase-8 activity. Concentrations of 25, 50 and 80 umol/L malvidin and delphinidin significantly disrupted mitochondrial permeability, showing anthocyanidins may be more effective at inducing apoptosis intrinsically. There was a significant interaction between anthocyanidin concentration and anthocyanidin type on cell viability, caspase-8 activity, and mitochondria permeability.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectAnthocyanidins
dc.subjectDelphinidin
dc.subjectMalvidin
dc.subjectCurcumin
dc.subjectApoptosis
dc.subjectCaspase-3
dc.subjectCaspase-8
dc.subjectHT-29 cells
dc.titleMalvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFoods and Nutrition
dc.description.majorFoods and Nutrition
dc.description.advisorJoan Fischer
dc.description.committeeJoan Fischer
dc.description.committeeArthur Grider
dc.description.committeeSilvia Giraudo


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