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dc.contributor.authorHariharan, Vani
dc.date.accessioned2014-03-04T21:12:35Z
dc.date.available2014-03-04T21:12:35Z
dc.date.issued2013-08
dc.identifier.otherhariharan_vani_201308_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/hariharan_vani_201308_ms
dc.identifier.urihttp://hdl.handle.net/10724/29056
dc.description.abstractGABA is a major inhibitory neurotransmitter in the central nervous system (CNS), which is synthesized from glutamate by the enzyme glutamic acid decarboxylase (GAD). There are two isoforms of GAD encoded by two genes gad1 and gad2. In mammals, gad1 encodes GAD67 and gad2 encodes GAD65. A decrease in GAD67 in the human brain is associated with schizophrenia and related neurological disorders. In mice, a loss of GAD65 causees spontaneous seizures. We studied the role of GAD in modulating neural activity in zebrafish. Splice blocking morpholinos were used to manipulate expression of gad in embryonic zebrafish. gad1-gad2 double morphants exhibited substantial reduction in GABA, leading to spontaneous seizures-like neural activity. Furthermore, gad1 and gad2 single morphants also exhibited seizure-like activity, suggesting that both GADs are important for the normal functioning of the brain.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectGABA
dc.subjectgad1
dc.subjectgad2
dc.subjectGAD67
dc.subjectGAD65
dc.subjectzebrafish
dc.subjectknockdown
dc.subjectsplice blocking morpholinos
dc.subjectneural activity
dc.subjectseizures
dc.subjectelectrophysiology
dc.titleDetermining the functions of gad1 and gad2 in embryonic zebrafish using splice blocking morpholinos
dc.typeThesis
dc.description.degreeMS
dc.description.departmentCellular Biology
dc.description.majorCellular Biology
dc.description.advisorJames D. Lauderdale
dc.description.committeeJames D. Lauderdale
dc.description.committeePing Shen
dc.description.committeeScott Dougan


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