Phenotyping methods and QTL mapping for late leaf spot resistance in cultivated peanut (Arachis hypogaea L.)
Gill, Rattandeep Kaur
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Late leaf spot (LLS), caused by Cercosporidium personatum, is a major foliar disease of peanut. LLS resistance is quantitative in nature. To identify genomic regions that control disease resistance, quantitative trait locus (QTL) mapping is required. Obtaining reliable phenotyping data is vital for mapping. Therefore, a population, consisting of 78 recombinant inbred lines (RILs) segregating for LLS resistance, was used for quantitative trait locus (QTL) analysis. Data were collected using three different phenotyping methods and comparisons were made. A set of 447 simple sequence repeat (SSR) and 25 single nucleotide polymorphism (SNP) markers was used for parental screening. The population was genotyped using 141 polymorphic loci. A linkage map with 94 loci, distributed into 19 LGs, was constructed based on the marker segregation data obtained from the population. A total of 7 QTL were identified that explained 10.9-38.4% of the variation in the phenotyping data.