Double eQTL mapping method to improve identification of trans eQTLs and construct intermediate gene networks
Sprolden, Tracy Lashonda
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A double eQTL mapping method for identifying trans eQTLs that reduces multiple testing and increases the statistical power of eQTL mapping is described. The first round of the method involves eQTL mapping for each gene using only the peak SNPs located on the same chromosome as the gene. All of the peak eQTLs found in the first round of mapping were cis eQTLs. Next the genes are clustered into similar expression groups and a list of combined eQTLs is created for the group. A final round of eQTL mapping is done for each gene using the list of combined eQTLs for the group. The eQTLs that are found as a result of the second eQTL mapping were trans eQTLs. The genes and trans eQTLs identified from the double eQTL mapping method were then used to construct intermediate gene networks that are thought to link causative loci and traits or diseases.