Virus origin influences transmission efficiency and frequency of amino acid exchanges in the glycoproteins of LPAIV islolates during serial passage in ducks
Ridenour, Callie Anne
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Studies focusing on evolution of low pathogenic avian influenza virus (LPAIV) are critical in understanding the continual circulation of these viruses. In this study, changes in the genetic composition of the hemagglutinin and neuraminidase glycoproteins as a result of transmission of LPAIV to a new host under experimental conditions were investigated. LPAIV isolates of chicken and wild bird origin were used to establish a serial passage transmission model in Pekin ducks. LPAIV isolates of divergent origin displayed differences in transmission capabilities and shedding patterns. Passage of the viruses in ducks resulted in a higher frequency of amino acid mutations in the hemagglutinin of the chicken isolate in comparison to the wild bird isolate. Relative genetic stability of the NA protein of both LPAIV isolates was observed after passage in ducks. Identifying the genetic adaptations surrounding transmission of LPAIV in new hosts may help to understand the selection contributing to the evolution and circulation of these viruses.