The effects of blueberry anthocyanidins on antioxidant enzyme activity in human HepG2 cells
Galambos, Alexis Rose
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Anthocyanins are thought to have antioxidant effects in the body. The effects of two anthocyanidins, malvidin and delphinidin, on the activities of antioxidant enzymes, glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-S-transferase (GST), and other markers of oxidative stress were examined in HepG2 human hepatocellular carcinoma cells. In study one, cells were treated with anthocyanidins at concentrations of 0, 5 or 10 µmol/L for 24 h and 0 or 200 µmol/L tert-butyl hydroperoxide (t-BOOH) for 2 h. Study two used a combined dose of 10 µmol/L anthocyanidins and 0 or 200 µmol/L t-BOOH. Cell proliferation and GR activity were significantly increased by delphinidin; with 5 μmol/L having the greatest effect in oxidatively stressed cells. Malvidin also increased cell proliferation, but to a lesser extent than delphinidin. No effect of treatment was observed on GST and GPx activity. Finally, it appeared that combining these anthocyanidins had an antagonistic effect.