The comparison of antibody- and ligand- coupled magnetic beads for the capture, recovery, and concentration of Norwalk virus-like particles and native GI.1 virus in buffer and food extract matrices
Freeman, Marlena Camille
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Norovirus is recognized as the principal cause of acute gastroenteritis in adults, causing over 90% of non-bacterial and an estimated 50% of all-cause epidemic gastroenteritis globally(Patel et al., 2008). There is a need for fast, accurate, reliable and cost-effective methods to detect norovirus in foods. The objective of this experiment was to evaluate the efficiency of HBGA ligand-coupled magnetic beads in capturing native GI.1 virus or norovirus virus-like particles (VLPs) in the context of common elution buffers and food residuals in order to determine their value as part of a norovirus detection technique. The data showed that the ligand coated magnetic bead capture method can be utilized for Norwalk VLP and native GI.1 virus, and also has the potential with further research and exploration to be a useful rapid method for the concentration and recovery of norovirus in both buffer and food matrices.