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dc.contributor.authorTeat, Stephanie Vande Ven
dc.date.accessioned2014-03-04T20:25:23Z
dc.date.available2014-03-04T20:25:23Z
dc.date.issued2011-12
dc.identifier.otherteat_stephanie_v_201112_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/teat_stephanie_v_201112_ms
dc.identifier.urihttp://hdl.handle.net/10724/27834
dc.description.abstractMycobacterium tuberculosis causes 1.4 million tuberculosis deaths annually. This bacterium has a complex, lipid-rich cell wall which facilitates resistance to killing by various caustic reagents. Experiments in this thesis examine aspects of mycobacterial physiology. One study assessed mycobacterial resistance to organic solvents. Equal volume 5-minute extraction with methanol reduced aqueous phase viability of M. tuberculosis by 4.5-log10 CFU, while acetone resulted in a reduction of 2-log10 CFU. Another study examined whether drying spilled M. tuberculosis on a solid surface was bacteriocidal. Results indicated only a 2-log10 CFU reduction after 12 hours. A third line of research was to develop vectors for deleting two M. tuberculosis genomic targets regulated by a virulence-associated sigma factor. Plasmids specific for each target were generated, but conversion into specialized transducing phages was unsuccessful. The fourth study indicated that this sigma factor did not contribute to bacterial antibiotic tolerance in macrophages.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectMycobacterium
dc.subjecttuberculosis
dc.subjectlaboratory safety
dc.subjectSigC
dc.subjectctpB
dc.subjectPPE1
dc.subjectRv0102
dc.subjectphage
dc.subjecttransduction
dc.titleSensitivity of mycobacteria to organic solvents, evaporation and antibiotics
dc.typeThesis
dc.description.degreeMS
dc.description.departmentInfectious Diseases
dc.description.majorVeterinary and Biomedical Sciences
dc.description.advisorRussell Karls
dc.description.advisorFrederick D. Quinn
dc.description.committeeRussell Karls
dc.description.committeeFrederick D. Quinn
dc.description.committeeVincent Starai
dc.description.committeeBarbara Reaves
dc.description.committeeEric LaFontaine


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