Show simple item record

dc.contributor.authorBottjen, Rachel Christine
dc.date.accessioned2014-03-04T19:58:09Z
dc.date.available2014-03-04T19:58:09Z
dc.date.issued2011-05
dc.identifier.otherbottjen_rachel_c_201105_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/bottjen_rachel_c_201105_ms
dc.identifier.urihttp://hdl.handle.net/10724/27067
dc.description.abstractThe Microplitis demolitor bracovirus (MdBV) genome contains a large protein tyrosine phosphatase (PTP) gene family that encodes four enzymes with intact catalytic domains, including ptp-H2. Here we report expression of PTP-H2 in Escherichia coli cells as non-fusion or thioredoxin fusion proteins. Expressed protein was localized to hemocytes, and the purified nonfusion form of PTP-H2 exhibited classical Michaelis-Menten kinetics. Overall, our results indicate that PTP-H2 is a functional protein, specifically expressed in MdBV-infected hemocytes. Like many PDV-carrying wasps, Microplitis demolitor inhibits development of its host, Pseudoplusia includens. Here I report that MdBV infection caused a persistent state of hypertrehalosemia, but reduced nutrient stores at a slower rate than starved larvae. Hemolymph trehalase activity was reduced during infection, and feeding cessation was not related to trypsin activity nor hypertrehalosemia. Overall, my results suggest MdBV causes alterations in metabolism apart from starvation, which prevent the host from achieving critical weight.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectpolydnavirus, phosphatase, enzyme kinetics, metabolism dysregulation
dc.titleCharacterization of the viral protein tyrosine phosphatase-H2 and the metabolic consequences of Microplitis demolitor bracoviral infection in Pseudoplusia includens
dc.typeThesis
dc.description.degreeMS
dc.description.departmentEntomology Graduate Program
dc.description.majorEntomology
dc.description.advisorMichael R. Strand
dc.description.committeeMichael R. Strand
dc.description.committeeKevin Clark
dc.description.committeeMark Brown


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record