Variation in RAPD-PCR patterns may not be attributable to genetic differences among Salmonella Enteritidis
Mathis, Demetrius Latron
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Salmonella enterica serovar Enteritidis (SE) is a major public health concern because of the foodborne illness it causes in the US every year. The source of the most recent outbreak of SE was found to be table eggs, and eggs have been a known source for this pathogen since its emergence in the 1950’s. The Centers for Disease Control and Prevention (CDC) uses PFGE to identify genetically related Salmonella strains, but the ability of pulsed-field gel electrophoresis (PFGE) to discriminate between epidemiologically unrelated SE isolates is limited due to their clonal nature. Random amplified polymorphic DNA (RAPD) PCR has been proposed as an alternative tool for distinguishing between SE isolates, but suffers from poor reproducibility. The goal of the described research was to determine whether increasing the PCR stringency would reduce the amount of randomness in SE RAPD DNA patterns.