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dc.contributor.authorMoresco, Kira Ann
dc.date.accessioned2014-03-04T18:57:06Z
dc.date.available2014-03-04T18:57:06Z
dc.date.issued2010-08
dc.identifier.othermoresco_kira_a_201008_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/moresco_kira_a_201008_ms
dc.identifier.urihttp://hdl.handle.net/10724/26731
dc.description.abstractGrowth parameters of low pathogenicity avian influenza viruses were examined in eight cell cultures and in three types of avian embryos. Virus detection was compared for samples collected from experimentally infected birds, using real-time reverse transcriptase polymerase chain reaction, embryonating chicken eggs, and five cell culture systems. Virus isolation from wild bird samples was compared using embryonating chicken, duck, and turkey eggs, as well as two cell culture systems. Virus isolation success was lowest using cell culture. Virus isolation was similar in embryonating chicken, duck, and turkey eggs. Embryonating chickens eggs had the highest virus isolation efficiency for avian influenza virus samples with cycle threshold of less than or equal to thirty seven. Embryonating turkey and duck eggs produced one hundred percent association between detection by real-time reverse transcription polymerase chain reaction and virus isolation up to cycle threshold of thirty six for avian paramyxovirus serotype 1. INDEX WORDS: Low pathogenicity avian influenza; Cell culture; Avian embryos; Real-time reverse transcription polymerase chain reaction
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectLow pathogenicity avian influenza
dc.subjectCell culture
dc.subjectAvian embryos
dc.subjectReal-time reverse transcription polymerase chain reaction
dc.titleEvaluation of avian embryos and cell culture for isolation and propagation of low pathogenicity avian influenza viruses
dc.typeThesis
dc.description.degreeMS
dc.description.departmentVeterinary Pathology
dc.description.majorVeterinary Pathology
dc.description.advisorDavid Swayne
dc.description.committeeDavid Swayne
dc.description.committeeDavid Stallknecht
dc.description.committeeHolly Sellers


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