Show simple item record

dc.contributor.authorHunt, Ryan Webster
dc.date.accessioned2014-03-04T18:56:16Z
dc.date.available2014-03-04T18:56:16Z
dc.date.issued2010-08
dc.identifier.otherhunt_ryan_w_201008_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/hunt_ryan_w_201008_ms
dc.identifier.urihttp://hdl.handle.net/10724/26662
dc.description.abstractThe influence of 12 biochemical stimulants, namely 2-phenylacetic acid (PAA; 30 ppm), indole-3 butyric acid (IBA; 10 ppm), 1- naphthaleneacetic acid (NAA; 2.5, 5 and 10 ppm ), gibberellic acid (GA3, 10 ppm), zeatin (ZT; 0.002 ppm), thidiazuron (TDZ; 0.22 ppm), humic acid (HA; 20 ppm), kelp extract (KE; 250 ppm), methanol (MeOH; 500 ppm), ferric chloride (FeCl3; 3.2 ppm), putrescine (PU; 0.09 ppm), spermidine (SPD; 1.5 ppm) were prescreened for their influence on growth and metabolites for the green alga- Chlorella sorokiniana. C. sorokiniana responded best to phytohormones in the auxin family, particularly NAA. Combinations of phytohormones were studied which compared blends from within the auxin family as well as against other families. The following study investigated the impact on biomass and chlorophyll productivity by comparing the delivery method of one of the top performing compounds shortlisted from prior research, the synthetic auxin naphthalene acetic-acid (NAA), solubilized by ethanol or methanol. This treatment was applied to on the green alga, Chlorella sorokiniana, as well as a mixed consortium that includes C. sorokiniana along with two other wild-isolated green algae, Scenedesmus bijuga and Chlorella minutissima. It was found that the use of ethanol to dissolve NAA was the most effective to boost the biomass productivity of C. sorokiniana, whereas, the mixed consortia did not demonstrate a dramatic beneficial response. The most effective treatment, EtOH500ppm+NAA5ppm, along with two other NAA concentrations (NAA2.5ppm and NAA5ppm) were then applied to six diverse species of microalgae to determine if the treatment dosage was effective for other freshwater and marine green algae, cyanobacteria, coccolithophore and diatoms. The use of ethanol and NAA at a combined dosage of EtOH500ppm+NAA5ppm was found to generate the highest biomass productivity for each of the species which responded positively to the treatments. If scalable, NAA and ethanol may have the potential to lower production costs by increasing biomass yields for commercial microalgae cultivation.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectPlant growth regulators, Phytohormones, Auxins
dc.subjectnaphthalene acetic acid
dc.subjectBioenergy
dc.subjectBiofuels
dc.subjectBiomass
dc.subjectBiostimulants
dc.subjectMicroalgae
dc.subjectPhytohormones
dc.subjectChlorella sorokiniana
dc.subjectHaematococcus pluvialis
dc.subjectPhaeodactylum tricornutum
dc.subjectPleurochrysis carterae
dc.subjectDunaliella
dc.titleBiochemical stimulation of microalgae for enhancing biomass productivity
dc.typeThesis
dc.description.degreeMS
dc.description.departmentBiological and Agricultural Engineering
dc.description.majorBiological Engineering
dc.description.advisorK.C. Das
dc.description.committeeK.C. Das
dc.description.committeeWilliam S. Kisaalita
dc.description.committeeMark A. Haidekker


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record