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dc.contributor.authorBaas, Sarah Ruth
dc.date.accessioned2014-03-04T18:26:19Z
dc.date.available2014-03-04T18:26:19Z
dc.date.issued2010-05
dc.identifier.otherbaas_sarah_r_201005_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/baas_sarah_r_201005_phd
dc.identifier.urihttp://hdl.handle.net/10724/26223
dc.description.abstractThe addition of carbohydrate structures (glycans) to proteins is an essential post-translational modification that begins in the endoplasmic reticulum and is completed through Golgi remodeling. Glycosylation can be cell-specific, tissue-specific, and developmental stage-specific. Altered glycosylation patterns are a hallmark of many cancers as well as many neuronal and muscular diseases, and developmental disorders. However, the mechanisms that regulate glycosylation in a tissue-specific manner are not well understood. A genetic screen in Drosophila melanogaster yielded a mutation called sugar-free frosting (sff), the Drosophila homolog of SAD kinase. Analysis of all of the N-linked glycans in sff mutant embryos revealed a shift towards more complex structures, a shift determined to stem from altered Golgi compartmentation. Many signaling pathways impinge on sff/SAD function, allowing the cellular microenvironment to exert minute control over the N-glycosylation patterns of neurons. One of those pathways receives signaling from a Toll-like receptor (Tollo) expressed on a neighboring non-neural cell. Pharmacological studies indicate that the signal relayed to the neural cell is a biogenic amine. In addition, White, which contributes to eye pigment biosynthesis, interacts with Sff/SAD and influences the phenotypes associated with the sff mutation. White also influences N-glycan expression. Therefore, White, biogenic amines, and Sugar-free frosting/SAD kinase, define nodes in a pathway that modulates neural-specific glycosylation in Drosophila.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectSAD kinase
dc.subjectglycosylation
dc.subjectHRP-epitope
dc.subjectGolgi
dc.subjectWhite
dc.subjectBiogenic amines
dc.titleThe regulation of neural-specific glycosylation in Drosophila melanogaster
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentBiochemistry and Molecular Biology
dc.description.majorBiochemistry and Molecular Biology
dc.description.advisorMichael Tiemeyer
dc.description.committeeMichael Tiemeyer
dc.description.committeeLance Wells
dc.description.committeeRichard Steet
dc.description.committeeMichael Pierce
dc.description.committeeStephen Dalton


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