Show simple item record

dc.contributor.authorCarte, Jason Michael
dc.date.accessioned2014-03-04T18:21:26Z
dc.date.available2014-03-04T18:21:26Z
dc.date.issued2009-12
dc.identifier.othercarte_jason_m_200912_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/carte_jason_m_200912_ms
dc.identifier.urihttp://hdl.handle.net/10724/26020
dc.description.abstractThe CRISPR-Cas sytem provides many prokaryotes with acquired resistance to genome invaders such as viruses and other mobile genetic elements. This system uses small RNAs derived from Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci encoded in the host genome as well as Cas (CRISPR-associated) proteins. We have identified an endoribonuclease, Cas6, that plays a role in CRISPR (cr)RNA biogenesis. Cas6 interacts with sequence elements in the 5’ region of precursor crRNAs and cleaves within the 3’ region of the repeat. The cleavage was found to be divalent metal ion independent and occurs on the 5’ side of the phosphodiester bond. Furthermore, the active site of Cas6 appears to consist of a triad of amino acids composed of conserved tyrosine, histidine, and lysine residues.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectCRISPR
dc.subjectCas
dc.subjectCas6
dc.subjectendoribonuclease
dc.subjectRNA
dc.subjectRNA processing
dc.subjectviral
dc.titleIdentification of Cas6 as an endoribonuclease involved in CRISPR-mediated genome defense in prokaryotes
dc.typeThesis
dc.description.degreeMS
dc.description.departmentBiochemistry and Molecular Biology
dc.description.majorBiochemistry and Molecular Biology
dc.description.advisorMichael Terns
dc.description.committeeMichael Terns
dc.description.committeeRebecca Terns
dc.description.committeeSidney Kushner
dc.description.committeeStephen Hajduk


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record