Show simple item record

dc.contributor.authorPennington, Catherine Aileen
dc.date.accessioned2014-03-04T18:16:39Z
dc.date.available2014-03-04T18:16:39Z
dc.date.issued2009-05
dc.identifier.otherpennington_catherine_a_200905_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/pennington_catherine_a_200905_ms
dc.identifier.urihttp://hdl.handle.net/10724/25608
dc.description.abstractLysR-type transcriptional regulators, BenM and CatM, tightly regulate the genes involved in aromatic compound degradation in Acinetobacter baylyi strain ADP1. BenM and CatM share similarities in protein sequence and promoter recognition, yet have striking differences in regulatory function. The experiments described here explore the regulatory role of CatM in the absence of a functional BenM. Spontaneous mutations in the catB promoter were identified in strains that maintained the ability to grow on benzoate as a sole carbon source in the absence of BenM. Characterization of the metabolism, gene expression, enzyme activity, and binding affinity of these mutants ultimately lead to a model of CatM-mediated expression of the benA promoter in response to increased intracellular concentrations of muconate.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectLysR-type transcriptional regulator, aromatic compound, promoter specificity, fluorescence anisotropy
dc.titleMutations in the catB operator-promoter region allow alternative regualtion of the benA promoter in Acinetobacter baylyi strain ADP1
dc.typeThesis
dc.description.degreeMS
dc.description.departmentMicrobiology
dc.description.majorMicrobiology
dc.description.advisorEllen Neidle
dc.description.committeeEllen Neidle
dc.description.committeeCory Momany
dc.description.committeeAnna Karls


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record