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dc.contributor.authorLu, Fang
dc.date.accessioned2014-03-04T18:15:58Z
dc.date.available2014-03-04T18:15:58Z
dc.date.issued2009-05
dc.identifier.otherlu_fang_200905_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/lu_fang_200905_ms
dc.identifier.urihttp://hdl.handle.net/10724/25549
dc.description.abstractDouble-strand break (DSB) repair is essential for cell survival and for the maintenance of genome integrity. In this study, we utilized I-Sce I, an endonuclease from yeast with an 18-bp recognition site to introduce DSB in maize and rice cells. We then employed PCR to characterize the inaccurate repair events that removed the I-Sce I recognition site. Of 82 and 84 inaccurate repair events characterized in maize and rice, more than 72% inaccurate repairs were associated with deletions. Of the deletion repairs, small deletions (1-9 nucleotides) happened more frequently than large deletions. The sequences flanking these deletions and insertions have the hallmarks of illegitimate recombination. These results are compatible with models suggesting that inaccurate repair of DSBs is the major factor responsible for the rapid removal of unselected DNA from higher plant genomes. The sizes of inaccurate repairs are not necessarily associated with genome size variation in monocot species.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectComparative genomics
dc.subjectDouble-strand breaks(DSBs)
dc.subjectI-SceI
dc.subjectMaize
dc.subjectRice
dc.titleA comparative genomic study of double-strand break repair in maize and rice
dc.typeThesis
dc.description.degreeMS
dc.description.departmentGenetics
dc.description.majorGenetics
dc.description.advisorJeff Bennetzen
dc.description.committeeJeff Bennetzen
dc.description.committeeMichael McEachern
dc.description.committeeR. Kelly Dawe


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