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dc.contributor.authorWiggins, Latoya Tameshi
dc.date.accessioned2014-03-04T16:22:50Z
dc.date.available2014-03-04T16:22:50Z
dc.date.issued2008-12
dc.identifier.otherwiggins_latoya_t_200812_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/wiggins_latoya_t_200812_ms
dc.identifier.urihttp://hdl.handle.net/10724/25358
dc.description.abstractConcerns over Listeria monocytogenes and its antimicrobial resistance are increasing for the food industry. Twenty isolates of L. monocytogenes were evaluated in this study for their resistance to three membrane perturbing antimicrobials - nisin, monensin, and gramicidin. Isolate ILSI NA 39, with similar susceptibilities to each antimicrobial, was further studied. The relationship of induced resistance to the other antimicrobials’ activity was determined by comparing growth of wild type and resistant strains in the presence of each antimicrobial at 10oC. Gramicidin manifested the lowest MIC against wild type and resistant ILSI NA 39 strains. Monensin was effective in agar MIC studies but not in broth studies. A slight genetic alteration (indicated by PFGE analysis) appeared in nisin resistant strains, while membrane protein differences (indicated by SDS-PAGE) occurred in gramicidin resistant strains. Further genomic, proteomic and lipid membrane studies are required to further describe relationships of the strains to the three antimicrobials.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectListeria monocytogenes
dc.subjectantimicrobial resistance
dc.subjectnisin
dc.subjectmonensin
dc.subjectgramicidin
dc.subjectsusceptibility
dc.subjectMIC
dc.subjectPFGE
dc.subjectSDS-PAGE
dc.titleRelationship of Listeria monocytogenes resistance to nisin and ionophore antibiotics
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFood Science and Technology
dc.description.majorFood Science
dc.description.advisorNorman Stern
dc.description.committeeNorman Stern
dc.description.committeeJinru Chen
dc.description.committeeJoseph Frank


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