• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Imaging intercellular calcium waves in the developing nervous system of zebrafish

    Thumbnail
    Date
    2008-08
    Author
    Srinivasamoorthy, Ganesh
    Metadata
    Show full item record
    Abstract
    Several critical developmental events in the brain are believed to be regulated by spontaneous calcium waves. Commonly used calcium imaging methodologies have not been successful in exploring calcium waves beyond the embryonic stages of zebrafish, due to their invasivity, high noise, poor spatial resolution and other problems. However, using zebrafish transgenic for Cameleon - a FRET based calcium indicator and SOARS - a statistical optimization technique for data analysis, we were able to overcome these limitations and were able to image spontaneous calcium waves in vivo in a live zebrafish brain. The zebrafish that we imaged stably expressed cameleon in all the neurons. Changes in the cameleon FRET signal were detected using SOARS, which is capable of detecting signals from noisy ratiometric datasets. Our results represent the first successful instance of imaging calcium waves with well preserved spatio-temporal information in a live larval zebrafish brain in vivo.
    URI
    http://purl.galileo.usg.edu/uga_etd/srinivasamoorthy_ganesh_200808_ms
    http://hdl.handle.net/10724/25058
    Collections
    • University of Georgia Theses and Dissertations

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback