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dc.contributor.authorRushing, Sarah
dc.date.accessioned2014-03-04T16:19:24Z
dc.date.available2014-03-04T16:19:24Z
dc.date.issued2008-08
dc.identifier.otherrushing_sarah_a_200808_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/rushing_sarah_a_200808_ms
dc.identifier.urihttp://hdl.handle.net/10724/25042
dc.description.abstractThe kinetochore is a highly conserved group of proteins involved in proper chromosome segregation by binding to the microtubules. Recent studies have identified more than 80 kinetochore components, many of which have a broad evolutionary conservation from yeast to humans. Among these are MCM21, NDC80, NUF2 and SPC25 that are predicted to be located in the core kinetochore regions and have been shown to have homologues within plants. Through mutant analysis by T-DNA and RNAi, and expression analysis using fluorescent fusion proteins, we attempted to characterize the function and localization of these proteins to further confirm their conservation in plants. We showed that SPC25 localizes to the kinetochore and is constitutively expressed throughout the tissues. It was also shown that mutation of SPC25 and NDC80 has adverse effects on the mutant plant’s development compared to wildtype plants. Based on our data, we suggest that MCM21 is not a conserved feature of the plant kinetochore and SPC25 and NDC80 are most likely conserved in plants. Due to questionable data, we were unable to make any conclusions regarding the location and function of NUF2.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectKinetochore
dc.subjectCentromere
dc.subjectArabidopsis thaliana
dc.subjectHTR12
dc.subjectNDC80
dc.subjectSPC25
dc.subjectMCM21
dc.subjectNUF2
dc.titleCharacterization of Arabidopsis thaliana kinetochore proteins
dc.typeThesis
dc.description.degreeMS
dc.description.departmentPlant Biology
dc.description.majorPlant Biology
dc.description.advisorR. Kelly Dawe
dc.description.committeeR. Kelly Dawe
dc.description.committeeJohn Burke
dc.description.committeeMichael Hahn


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