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dc.contributor.authorNorris, Michelle Beth
dc.date.accessioned2014-03-04T03:27:55Z
dc.date.available2014-03-04T03:27:55Z
dc.date.issued2008-08
dc.identifier.othernorris_michelle_b_200808_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/norris_michelle_b_200808_ms
dc.identifier.urihttp://hdl.handle.net/10724/25013
dc.description.abstractWe used transgenic medaka to evaluate whether analysis of mutations directly in isolated spermatozoa is a reliable alternative to using progeny to assess genetic health risks. Mutant frequencies of cII targets (MF) in spermatozoa exposed to ethylnitrosurea (ENU) at either post-meiotic or pre-meiotic germ-cell stages of spermatogenesis were compared. The MFs of exposed pre-meiotic stem cell spermatogonia showed a significant 9-fold induction in MF, consistent with progeny analyses. By contrast, significantly elevated MFs were detected in progeny derived from ENU-treated post-meiotic germ cells, but not in isolated spermatozoa. DNA damage not fixed as a cII mutation in spermatozoa will not be detected. However, damage in spermatozoa that persists to or after fertilization can be fixed as a mutation and is detected in the embryo. Consequently, using isolated spermatozoa can provide a reliable alternative to using progeny providing the spermatozoa sampled correspond to pre-meiotic germ-cell stages exposed to the mutagen.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectGerm cells
dc.subjectSpermatozoa
dc.subjectcII mutation target gene
dc.subjectMutant frequency
dc.titleIsolated spermatozoa as indicators of mutations transmitted to progeny
dc.typeThesis
dc.description.degreeMS
dc.description.departmentForest Resources
dc.description.majorToxicology
dc.description.advisorRichard N. Winn
dc.description.committeeRichard N. Winn
dc.description.committeeDon G. Ennis
dc.description.committeeTravis C. Glenn


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